Much Better Gluten Quantitation by GP-HPLC-FLD October 14, 2016Posted by stuffilikenet in Awesome, Science.
Katharina Anne Scherf*, Herbert Wieser, and Peter Koehler combined gel-permeation high-performance liquid chromatography with fluorescence detection to develop a sensitive technique that can detect both gliadins and glutenins in purified wheat starch. The new method identified higher amounts of gluten in 19 out of 26 samples than the ELISA analyses did. And, according to the new test, 12 samples that had been labeled gluten-free contained between 25.6 and 69 milligrams of gluten per kilogram of starch. The U.S. Food and Drug Administration and the United Nations’ Food and Agriculture Organization have set the maximum limit for gluten in products labeled gluten-free at 20 mg/kg.
Homework: Improved Quantitation of Gluten in Wheat Starch for Celiac Disease Patients by Gel-Permeation High-Performance Liquid Chromatography with Fluorescence Detection (GP-HPLC-FLD), Katharina Anne Scherf*, Herbert Wieser, and Peter Koehler, Deutsche Forschungsanstalt für Lebensmittelchemie, Leibniz Institut, Lise-Meitner-Straße 34, 85354 Freising, Germany J. Agric. Food Chem., 2016, 64 (40), pp 7622–7631. DOI: 10.1021/acs.jafc.6b02512